Chloroplast transformation in chlamydomonas with high velocity microprojectiles pdf

2019-08-18 20:45

The Argininosuccinate Lyase Gene of Chlamydomonas reinhardtii Is an Important Tool for Nuclear Transformation and for Correlating the Genetic and Molecular Maps of the arg7 Locus, EMBO J. , 1989, vol. 8, pp. .Abstract Bombardment of three mutants of the chloroplast atpB gene of Chlamydomonas reinhardtii with highvelocity tungsten microprojectiles that were coated with cloned chloroplast DNA carrying the wildtype gene permanently restored the photosynthetic capacity of the algae. chloroplast transformation in chlamydomonas with high velocity microprojectiles pdf

The restored wildtype atpB gene remains in all transformants as an integral part of the chloroplast genome and is expressed and inherited normally.

Stable inheritance and sustainedhigh level expression of foreign genes in the progeny are the most critical factors for successful application of genetic engineering in agriculture. In this study, we have transformed cry 1Ia5 into tobacco chloroplasts and studied the expression, inheritance and resistance offered against Helicoverpa armigera over two generations. Chloroplast transformation in the photosynthetic alga Chlamydomonas reinhardtii has been used to explore the potential to use it as an inexpensive and easily scalable system for the production of therapeutic recombinant proteins. Diverse proteins, such as bacterial and viral antigens, antibodies and, immunotoxins have been successfully expressed in the chloroplast using endogenous and chimeric chloroplast transformation in chlamydomonas with high velocity microprojectiles pdf Studies on Chlamydomonas chloroplast transformation: foreign DNA can be stably maintained in the chromosome. Plant Cell. 1989 Jan; 1 (1): . [PMC free article Kindle KL, Schnell RA, Fernndez E, Lefebvre PA. Stable nuclear transformation of Chlamydomonas using the Chlamydomonas gene for nitrate reductase.

Studies on Chlamydomonas Chloroplast Transformation: Foreign DNA Can Be Stably Maintained in the highvelocity microprojectiles (Klein et al. , utilized this mutant as the recipient strain for highvelocity microprojectile transformation of the C. reinhardtii chloro plast because of its strong selectable phenotype (restora chloroplast transformation in chlamydomonas with high velocity microprojectiles pdf Kindle KL. Highfrequency nuclear transformation of Chlamydomonas reinhardtii. Proc Natl Acad Sci U S A. 1990 Feb; 87 (3): . [PMC free article Kindle KL, Schnell RA, Fernndez E, Lefebvre PA. Stable nuclear transformation of Chlamydomonas using the Chlamydomonas gene for nitrate reductase. J Cell Biol. 1989 Dec; 109 (6 Pt 1): . Boynton JE, Gillham NW, Harris EH, Hosler JP, Johnson AM, Jones AR, RandolphAnderson BL, Robertson D, Klein TM, Shark KB, et al. Chloroplast transformation in Chlamydomonas with high velocity microprojectiles. Recombination of Chlamydomonas chloroplast DNA occurs more frequently in the large inverted repeat sequence than in the singlecopy regions. RandolphAnderson BL, Robertson D, Klein TM, Shark KB, Sanford JC (1988) Chloroplast transformation in Chlamydomonas with high velocity microprojectiles. Bombardment of three mutants of the chloroplast atpB gene of Chlamydomonas reinhardtii with highvelocity tungsten microprojectiles that were coated with cloned chloroplast DNA carrying the wildtype gene permanently restored the photosynthetic capacity of the algae.

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